Sarcosine Oxidase (SOD-TE) ゼルコシンオキシダーゼ(SOD-E)

  • 臨床診断用酵素

臨床検査において、クレアチンおよびクレアチニンの測定に利用されます。

由来 recombinant E. coli
系統名

Sarcosine : oxygen oxidoreductase (demethylating)

EC 番号 1.5.3.1
反応式

Sarcosine + H2O + O2 →→→ Glycine + Formaldehyde + H2O2

仕様

Appearance yellow lyophilizate
Activity ≧25 U/mg lyophilizate
Contaminants catalase  <0.5%
glucose oxidase <1.0×10-5
Stabilizer sucrose 
Storage below -20℃ protected from light

特性

Molecular weight ca. 49 kDa (gel filtration)
Structure monomer of 43 kDa (SDS-PAGE)
one mole of FAD per mole of enzyme
Isoelectric point 5.3
Michaelis constant 4.7×10-3M (sarcosine)
pH Optimum 6.7–9.5
pH Stability 6.5–10.5
Optimum temperature 50℃
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Zn2+,Cu2+,Hg2+,Ag+

アプリケーション

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

参考文献

Suzuki, M. (1979)
クレアチニンの酵素的測定法
Medical Technology, 7, 945–950.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

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