臨床検査および連続乳酸測定センサにおいて、乳酸の測定に利用されます。
| 由来 | recombinant E. coli |
|---|---|
| 系統名 | (s)-Lactate : ferricytochrome-c 2-oxidoreductase |
| EC 番号 | 1.1.2.3 |
| 反応式 | L-Lactate + acceptor → → → Pyruvate + reduced acceptor |
Lactate dehydrogenase (LDH-E) ラクテートデヒドロゲナーゼ(LDH-E)
- 臨床診断用酵素
仕様
| Appearance | Reddish brown lyophilizate | |
|---|---|---|
| Activity | ≧230 U/mg | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 55 kDa (SDS-PAGE) |
|---|---|
| Structure | tetramer |
| Coenzymes (Cofactors) | FMN, Heme b (protoheme IX) |
| Michaelis constant | 1.7×10-3M (L-Lactate) |
| pH Optimum | 7.5 |
| pH Stability | 7.0–8.0 |
| Optimum temperature | 40℃ |
| Thermal stability (liquid form) | below 40℃ |
アプリケーション
The enzyme is useful for the determination of lactate in clinical analysis and continuous lactate monitoring sensor.