臨床検査において、α-アミラーゼおよび無機リン酸の測定に利用されます。
| 由来 | recombinant E. coli |
|---|---|
| 系統名 | β-D-Glucose 1,6-phosphomutase |
| EC 番号 | 5.4.2.6 |
| 反応式 | β-D-Glucose 1-phosphate →→→ β-D-Glucose 6-phosphate |
beta-Phosphoglucomutase (βPGM-EP) β-ホスホグルコムターゼ(βPGM-EP)
- 臨床診断用酵素
仕様
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧30 U/mg | |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA) | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 34 kDa (gel filtration) |
|---|---|
| Structure | monomer of ca. 25 kDa (SDS-PAGE) |
| Michaelis constant | 2.3×10-4M (β-D-glucose-1-phosphate) |
| pH Optimum | ca. 7.0 |
| pH Stability | 5.0–9.5 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at lest one month |
| Activators | Mg2+，Mn2+，Co2+，Ni2+ |
| Inhibitors |
Hg2+，Zn2+，Cu2+，Cd2+ |
アプリケーション
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
参考文献
- Nakamura, K. et al., J. Ferment. Bioeng., 85, 350–353 (1998).
- Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).