beta-Phosphoglucomutase (bPGM-EP) β-ホスホグルコムターゼ(βPGM-EP)

  • 臨床診断用酵素

臨床検査において、α-アミラーゼおよび無機リン酸の測定に利用されます。

由来 recombinant E. coli
系統名

β-D-Glucose 1,6-phosphomutase

EC 番号 5.4.2.6
反応式

β-D-Glucose 1-phosphate →→→ β-D-Glucose 6-phosphate

仕様

Appearance white lyophilizate
Activity ≧30 U/mg lyophilizate
Contaminants NADH oxidase ≦5.0×10-4
6-phosphogluconate dehydrogenase ≦5.0×10-4
Stabilizer lactose, EDTA
Storage below - 20℃

特性

Molecular weight ca. 34 kDa (gel filtration)
Structure monomer of ca. 25 kDa (SDS-PAGE)
Michaelis constant 2.3×10-4M (β-D-glucose-1-phosphate)
pH Optimum ca. 7.0
pH Stability 5.0–9.5
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at lest one month
Activators Mg2+,Mn2+,Co2+,Ni2+
Inhibitors

Hg2+,Zn2+,Cu2+,Cd2+

アプリケーション

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

参考文献

  • Nakamura, K. et al., J. Ferment. Bioeng., 85, 350–353 (1998).
  • Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).

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