beta-Phosphoglucomutase (βPGM-EP) β- 磷酸葡糖变位酶

  • 临床检测用酶

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

由来 recombinant E. coli
系统名称

β-D-Glucose 1,6-phosphomutase

EC编号 5.4.2.6
反应式

β-D-Glucose 1-phosphate →→→ β-D-Glucose 6-phosphate

规格

Appearance white lyophilizate
Activity ≧30 U/mg
Stabilizer lactose,  ethylenediaminetetraacetic acid (EDTA)
Storage condition below -20℃

特性

Molecular weight ca. 34 kDa (gel filtration)
Structure monomer of ca. 25 kDa (SDS-PAGE)
Michaelis constant 2.3×10-4M (β-D-glucose-1-phosphate)
pH Optimum ca. 7.0
pH Stability 5.0–9.5
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at lest one month
Activators Mg2+,Mn2+,Co2+,Ni2+
Inhibitors

Hg2+,Zn2+,Cu2+,Cd2+

适用类型

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

参考文献

  • Nakamura, K. et al., J. Ferment. Bioeng., 85, 350–353 (1998).
  • Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).

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