Fructosyl-peptide Oxidase (FPOX-CET) フルクトシルペプチドオキシダーゼ(FPOX-CET)

  • 臨床診断用酵素

臨床検査において、フルクトシル-L-アミノ酸およびフルクトシルペプチドの測定に利用されます。

由来 recombinant E. coli
系統名

Fructosyl-peptide : oxygen oxidoreductase

EC 番号 1.5.3
反応式

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

仕様

Appearance yellow lyophilizate
Activity ≧5.0 U/mg lyophilizate
Stabilizer glutamate, EDTA
Storage below -20℃ protected from light

特性

Molecular weight ca. 60 kDa (gel filtration)
Structure monomer of 52 kDa(SDS-PAGE)
Michaelis constant 1.5×10-3M(fructosyl-valyl-histidine)
5.0×10-3M (fructosyl-glysine)
9.0×10-3M(Nε-fructosyl-lysine)
pH Optimum 7.5–8.5
pH Stability 5.5–9.5
Optimum temperature 37–45℃
Thermal stability below 45℃
Stability (powder form) stable at -20℃ for at least 12 months
Inhibitors Hg2+ ,Pb2+
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine(150)
Nε-fructosyl-L-lysine (68.6)

アプリケーション

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

参考文献

Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.

Hirokawa K, Ichiyanagi A, Kajiyama N. (2008)
Enhancement of thermostability of fungal deglycating enzymes by directed evolution.
Applied Mcrobiology and Biotechnology, 78(5), 775-781.

Ichiyanagi A, Hirokawa K, Gomi K, Nakatsu T, Kato H, Kajiyama N. (2013)
Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(2), 130-133.

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