Glutaminase(GLN) グルタミナーゼ

  • 臨床診断用酵素

臨床検査において、L-グルタミンの測定に利用されます。

由来 from microorganism
系統名

L-Glutamine amidohydrolase

EC 番号 3.5.1.2
反応式

L-Glutamine + H2O →→→ L-Glutamineate + NH3

仕様

Appearance light gray lyophilizate
Activity ≧4 U/mg
Contaminants Catalase ≦3.0 U/U%
Stabilizer sucrose
Storage condition below - 20℃

特性

Molecular weight ca. 58 kDa (gel filtration)
Structure heterodimer (45 kDa and 22 kDa) (SDS-PAGE)
Michaelis constant  5.5×10-4 M (L-glutamine)
pH Optimum ca. 7.0
pH Stability 5.5–9.0
Optimum temperature ca. 65℃
Thermal stability below 50℃
Stability (liquid form) stable at 37℃ for at least four days
Stability (powder form) stable at 30℃ for at least two weeks
Specificity L-glutamine (100), D-glutamine (70), L-asparagine (0), D-asparagine (0)

アプリケーション

The enzyme is useful for the determination of L-glutamine in clinical analysis.

参考文献

Roberts, E., “The Enzymes,” Vol. 4 (2nd ed.), Academic Press, New York and London, 1960, pp. 285–300.

Hartman, S. C., “The Enzymes,” Vol. 4 (3rd ed.), Academic Press, New York and London, 1971, pp. 79–100.

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