Creatininase (C1- E) クレアチニナーゼ(C1-E)

  • 臨床診断用酵素

臨床検査において、クレアチニンの測定に利用されます。

由来 recombinant E. coli
系統名

Creatinine amidohydrolase

EC 番号 3.5.2.10
反応式

Creatinine H2O Creatine

仕様

Appearancewhite lyophilizate
Activity600-750 U/mg lyophilizate
Contaminantcatalase ≦0.5%
Stabilizersucrose
Storagebelow -20℃

特性

Molecular weightca. 170 kDa (gel filtration)
Structure 6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point4.8
Michaelis constant3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum6.5–7.0
pH Stability7.0–11.0
Optimum temperature 60–65℃
Thermal stabilitybelow 60℃
Stability (liquid form)stable at 37°C for at least two weeks 
Stability (powder form)stable at 30°C for at least one month
InhibitorsHg2+
ActivatorsMg2+,Mn2+ 

アプリケーション

The enzyme is useful for the determination of creatinine in clinical analysis.

参考文献

Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).

TOP