Creatininase (C1-E) クレアチニナーゼ(C1-E)

  • 臨床診断用酵素

臨床検査において、クレアチニンの測定に利用されます。

由来 recombinant E. coli
系統名

Creatinine amidohydrolase

EC 番号 3.5.2.10
反応式

Creatinine H2O Creatine

仕様

Appearance white lyophilizate
Activity 600-750 U/mg lyophilizate
Contaminant catalase ≦0.5%
Stabilizer sucrose
Storage below -20℃

特性

Molecular weight ca. 170 kDa (gel filtration)
Structure  6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature  60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

アプリケーション

The enzyme is useful for the determination of creatinine in clinical analysis.

参考文献

Suzuki, M. (1979)
クレアチニンの酵素的測定法
Medical Technology, 7, 945–950.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

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