Creatininase (C1- E)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine in clinical analysis.

Origin recombinant E. coli
Lineage name

Creatinine amidohydrolase

EC Number 3.5.2.10
Reaction formula

Creatinine H2O Creatine

SPECIFICATION

Appearance yellow lyophilizate
Activity ≧500 U/mg lyophilizate
Contaminant catalase ≦1.0%
Stabilizer sucrose
Storage below -20℃

PROPERTIES

Molecular weight ca. 170 kDa (gel filtration)
Structure  6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature  60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

APPLICATIONS

The enzyme is useful for the determination of creatinine in clinical analysis.

REFERENCES

Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).

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