Creatininase (C1- E)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine in clinical analysis.

Origin recombinant E. coli
Systematic name

Creatinine amidohydrolase

EC Number 3.5.2.10
Reaction formula

Creatinine H2O Creatine

SPECIFICATION

Appearanceyellow lyophilizate
Activity≧500 U/mg lyophilizate
Contaminantcatalase ≦1.0%
Stabilizersucrose
Storagebelow -20℃

PROPERTIES

Molecular weightca. 170 kDa (gel filtration)
Structure 6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point4.8
Michaelis constant3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum6.5–7.0
pH Stability7.0–11.0
Optimum temperature 60–65℃
Thermal stabilitybelow 60℃
Stability (liquid form)stable at 37°C for at least two weeks 
Stability (powder form)stable at 30°C for at least one month
InhibitorsHg2+
ActivatorsMg2+,Mn2+ 

APPLICATIONS

The enzyme is useful for the determination of creatinine in clinical analysis.

REFERENCES

Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).

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