Fructosyl-peptide Oxidase (FPOX-CE) フルクトシルペプチドオキシダーゼ(FPOX-CE)

  • 臨床診断用酵素

臨床検査において、フルクトシル-L-アミノ酸およびフルクトシルペプチドの測定に利用されます。

由来 recombinant E. coli
系統名

Fructosyl-peptide : oxygen oxidoreductase

EC 番号 1.5.3
反応式

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

仕様

Appearance yellow lyophilizate
Activity

≧6.0 U/mg lyophilizate

Stabilizer EDTA, glutamate
Storage below -20℃ protected from light

特性

Molecular weight ca. 60kDa (gel filtration)
Structure monomer of 52 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 3.4×10-4M (fructosyl-valyl-histidine)
4.4×10-4M (fructosyl-glycine)
8.9×10-4M (Nε-fructosyl-lysine)
pH Optimum 7.5–8.0
pH Stability 6.0–9.5
Optimum temperature 35–42℃
Thermal stability below 45℃
Stability (powder form) stable at 37℃ for at least one month
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

アプリケーション

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

参考文献

Hirokawa K, Gomi K, Bakke M, Kajiyama N. (2003)
Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi.
Archives of Microbiology, 180, 227-231.

Hirokawa K, Gomi K, Kajiyama N. (2003)
Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein.
Biochem Biophys Res Commun, 311(1), 104-111.

Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.

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