Fructosyl-peptide Oxidase (FPOX-CE) フルクトシルペプチドオキシダーゼ(FPOX-CE)

  • 臨床診断用酵素

臨床検査において、フルクトシル-L-アミノ酸およびフルクトシルペプチドの測定に利用されます。

由来 recombinant E. coli
系統名

Fructosyl-peptide : oxygen oxidoreductase

EC 番号 1.5.3
反応式

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

仕様

Appearance yellow lyophilizate
Activity

≧6.04 U/mg lyophilizate

Stabilizer EDTA, glutamate
Storage below -20℃ protected from light

特性

Molecular weight ca. 60kDa (gel filtration)
Structure monomer of 52 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 3.4×10-4M (fructosyl-valyl-histidine)
4.4×10-4M (fructosyl-glycine)
8.9×10-4M (Nε-fructosyl-lysine)
pH Optimum 7.5–8.0
pH Stability 6.0–9.5
Optimum temperature 35–42℃
Thermal stability below 45℃
Stability (powder form) stable at 37℃ for at least one month
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

アプリケーション

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

参考文献

  • Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
  • Hirokawa, K. et al., Arch. Microbiol., 180, 227–231 (2003).
  • Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104–111 (2003).

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