Fructosyl-peptide Oxidase (FPOX-CE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

Origin recombinant E. coli
Systematic name

Fructosyl-peptide : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

SPECIFICATION

Appearanceyellow lyophilizate
Activity

≧6.04 U/mg lyophilizate

StabilizerEDTA, glutamate
Storagebelow -20℃ protected from light

PROPERTIES

Molecular weightca. 60kDa (gel filtration)
Structuremonomer of 52 kDa (SDS-PAGE)
Isoelectric point4.2
Michaelis constant3.4×10-4M (fructosyl-valyl-histidine)
4.4×10-4M (fructosyl-glycine)
8.9×10-4M (Nε-fructosyl-lysine)
pH Optimum7.5–8.0
pH Stability6.0–9.5
Optimum temperature35–42℃
Thermal stabilitybelow 45℃
Stability (powder form)stable at 37℃ for at least one month
Specificityfructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

REFERENCES

  • Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
  • Hirokawa, K. et al., Arch. Microbiol., 180, 227–231 (2003).
  • Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104–111 (2003).

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