Sarcosine Oxidase (SOD-TE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

Origin recombinant E. coli
Systematic name

Sarcosine : oxygen oxidoreductase (demethylating)

EC Number 1.5.3.1
Reaction formula

Sarcosine + H2O + O2 →→→ Glycine + Formaldehyde + H2O2

SPECIFICATION

Appearanceyellow lyophilizate
Activity≧25 U/mg lyophilizate
Contaminantscatalase  <0.5%
glucose oxidase <1.0×10-5
Stabilizersucrose 
Storagebelow -20℃ protected from light

PROPERTIES

Molecular weightca. 49 kDa (gel filtration)
Structuremonomer of 43 kDa (SDS-PAGE)
one mole of FAD per mole of enzyme
Isoelectric point5.3
Michaelis constant4.7×10-3M (sarcosine)
pH Optimum6.7–9.5
pH Stability6.5–10.5
Optimum temperature50℃
Thermal stabilitybelow 55℃
Stability (liquid form)stable at 37℃ for at least two weeks
Stability (powder form)stable at 30℃ for at least one month
InhibitorsZn2+,Cu2+,Hg2+,Ag+

APPLICATIONS

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

REFERENCES

  • Suzuki, M., Medical Technology, 7, 945–950 (1979).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).
  • Koyama, Y. et al., Agric. Biol. Chem., 55, 1259–1263 (1991).

TOP