Sucrose Phosphorylase (SPL-E)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

Origin recombinant E. coli
Systematic name

Sucrose : orthophosphate α-D-glucosyltransferase

EC Number 2.4.1.7
Reaction formula

Sucrose + Orthophosphate →→→ D-Fructose + α-D-Glucose 1-phosphate

SPECIFICATION

Appearancewhite lyophilizate
Activity≧50 U/mg lyophilizate
ContaminantsNADH oxidase ≦5.0×10-4
6-phosphogluconate dehydrogenase ≦5.0×10-4
Stabilizersucrose
Storageat-20℃

PROPERTIES

Molecular weightca. 56 kDa (gel filtration)
Structuremonomer of 56 kDa (SDS-PAGE)
Isoelectric point4.6
Michaelis constant3.9×10-2M (sucrose)
6.2×10-3M (phosphate)
pH Optimum7.5
pH Stability5.0–8.0
Optimum temperature40℃
Thermal stabilitybelow 45℃
Stability (liquid form)stable at 37℃ for at least two weeks
Stability (powder form)stable at 30℃ for at least two weeks
Inhibitorsglucose, glucono-1,5-lactone
Specificitysucrose (100), maltose (0), starch (0)

APPLICATIONS

The enzyme is useful for the determination of inorganic phosphate in clinical analysis.

REFERENCES

  • Koga, T. et al., Agric. Biol. Chem., 55, 1805–1810 (1991).
  • Kitao, S. and Nakano, E., J. Ferment. Bioeng., 73, 179–184 (1992).

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