beta-Phosphoglucomutase (bPGM-EP)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

Origin recombinant E. coli
Lineage name

β-D-Glucose 1,6-phosphomutase

EC Number 5.4.2.6
Reaction formula

β-D-Glucose 1-phosphate →→→ β-D-Glucose 6-phosphate

SPECIFICATION

Appearance white lyophilizate
Activity ≧10 U/mg lyophilizate
Contaminants NADH oxidase ≦5.0×10-4
6-phosphogluconate dehydrogenase ≦2.0×10-4
Stabilizer lactose,EDTA
Storage below -20℃

PROPERTIES

Molecular weight

ca. 220 kDa (gel filtration)

Structure 2 subunits of 90 kDa (SDS-PAGE)
Michaelis constant

1.9×10-3M (phosphate)

3.4×10-3 M (phosphate)

8.3×10-3M (arsenate)

pH Optimum 6.5–7.5
pH Stability 5.5–8.0
Optimum temperature 45–50°C
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at least four weeks
Inhibitors

Hg2+,Ag+,Zn2+,Cu2+

APPLICATIONS

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

REFERENCES

  • Nakamura, K. et al., J. Ferment. Bioeng., 85, 350–353 (1998).
  • Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).

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