The enzyme is useful for the determination of lactate in clinical analysis and continuous lactate monitoring sensor.
| Origin | recombinant E. coli |
|---|---|
| Systematic name | (s)-Lactate : ferricytochrome-c 2-oxidoreductase |
| Reaction formula | L-Lactate + acceptor → → → Pyruvate + reduced acceptor |
| Appearance | Reddish brown lyophilizate | |
|---|---|---|
| Activity | ≧230 U/mg | |
| Storage condition | below -20℃ protected from light |
| Molecular weight | ca. 55 kDa (SDS-PAGE) |
|---|---|
| Structure | tetramer |
| Coenzymes (Cofactors) | FMN, Heme b (protoheme IX) |
| Michaelis constant | 1.7×10-3M (L-Lactate) |
| pH Optimum | 7.5 |
| pH Stability | 7.0–8.0 |
| Optimum temperature | 40℃ |
| Thermal stability (liquid form) | below 40℃ |
The enzyme is useful for the determination of lactate in clinical analysis and continuous lactate monitoring sensor.
