Uricase (U-TE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of uric acid in clinical analysis.

Origin recombinant E. coli
Systematic name

Urate : oxygen oxidoreductase

EC Number 1.7.3.3
Reaction formula

Urate + H2O + O2 →→→ Unidentified products + H2O2

             ↓

             CO2 + Allantoin (racemic mixture)

SPECIFICATION

Appearancelight brownish lyophilizate
Activity≧4U/mg lyophilizate
Contaminantcatalase ≦1.0%
Stabilizersucrose,citrate,EDTA
Storagebelow -20℃

PROPERTIES

Molecular weightca. 90 kDa (gel filtration)
Structure2 subunits of 35 kDa (SDS-PAGE)
Michaelis constant1.1×10-5M (uric acid)
pH Optimum8.5
pH Stability7.0–11.0
Optimum temperature45℃
Thermal stabilitybelow 55℃
Stability (liquid form)stable at 37℃ for at least ten days
Stability (powder form)stable at 30℃ at least three weeks
InhibitorsHg2+,Ag+

APPLICATIONS

The enzyme is useful for the determination of uric acid in clinical analysis.

REFERENCES

Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).

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