The enzyme is useful for the determination of uric acid in clinical analysis.
| Origin | recombinant E. coli |
|---|---|
| Systematic name | Urate : oxygen oxidoreductase |
| EC Number | 1.7.3.3 |
| Reaction formula | Urate + H2O + O2 →→→ Unidentified products + H2O2 ↓ CO2 + Allantoin (racemic mixture) |
| Appearance | light brownish lyophilizate | |
|---|---|---|
| Activity | ≧4.0 U/mg | |
| Contaminants | Catalase ≦1.0 U/U% | |
| Stabilizer | sucrose, citrate, ethylenediaminetetraacetic acid (EDTA) | |
| Storage condition | below -20℃ |
| Molecular weight | ca. 90 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 35 kDa (SDS-PAGE) |
| Michaelis constant | 1.1×10-5M (uric acid) |
| pH Optimum | 8.5 |
| pH Stability | 7.0–11.0 |
| Optimum temperature | 45℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least ten days |
| Stability (powder form) | stable at 30℃ at least three weeks |
| Inhibitors | Hg2+，Ag+ |
The enzyme is useful for the determination of uric acid in clinical analysis.
Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).
