Creatinase (C2-AE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

Origin recombinant E. coli
Systematic name

Creatine amidinohydrolase

EC Number 3.5.3.3
Reaction formula

Creatine + H2O →→→ Sarcosine + Urea

SPECIFICATION

Appearancewhite lyophilizate
Activity≧9 U/mg lyophilizate
Contaminantcatalase       <0.5%
Stabilizersucrose
Storagebelow -20℃

PROPERTIES

Molecular weightca. 80 kDa (gel filtration)
Structure2 subunits of 46 kDa (SDS-PAGE)
Michaelis constant1.3×10-2M (creatine)
pH Optimum7.0–9.0
pH Stability5.0–11.0
Optimum temperature40℃
Thermal stabilitybelow 45℃
Stability (liquid form)stable at 37℃ for at least one month
Stability (powder form)stable at 30℃ for at least one month
InhibitorsHg2+

APPLICATIONS

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

REFERENCES

  • Suzuki, M, Medical Technology, 7, 945–950 (1979).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).

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