Creatinase (C2-AE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

Origin recombinant E. coli
Lineage name

Creatine amidinohydrolase

EC Number 3.5.3.3
Reaction formula

Creatine + H2O →→→ Sarcosine + Urea

SPECIFICATION

Appearance white lyophilizate
Activity ≧9 U/mg lyophilizate
Contaminant catalase       <0.5%
Stabilizer sucrose
Storage below -20℃

PROPERTIES

Molecular weight ca. 80 kDa (gel filtration)
Structure 2 subunits of 46 kDa (SDS-PAGE)
Michaelis constant 1.3×10-2M (creatine)
pH Optimum 7.0–9.0
pH Stability 5.0–11.0
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least one month
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Hg2+

APPLICATIONS

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

REFERENCES

  • Suzuki, M, Medical Technology, 7, 945–950 (1979).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
  • Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).

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