Lactate Dehydrogenase (LDH-P)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

Origin porcine heart
Lineage name

(S )-Lactate : NAD+ oxidoreductase

EC Number 1.1.1.27
Reaction formula

Pyruvate + NADH + H+ L-Lactate + NAD+

SPECIFICATION

Appearance white lyophilizate
Activity ≧150 U/mg lyophilizate
Contaminants GPT ≦1.0×10-2
malate dehydrogenase ≦5.0×10-2
pyruvate kinase ≦1.0×10-2
Storage below -20℃

PROPERTIES

Molecular weight ca. 115 kDa (gel filtration)
Michaelis constant 2.5×10-2M (fructosyl-glycine)
1.0×10-4M (pyruvate)
pH Optimum 7.4–8.0
pH Stability 7.0–7.5
Optimum temperature 50–55℃
Thermal stability below 45℃

APPLICATIONS

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

REFERENCES

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).

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