Lactate Dehydrogenase (LDH-P)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

Origin porcine heart
Systematic name

(S )-Lactate : NAD+ oxidoreductase

EC Number 1.1.1.27
Reaction formula

Pyruvate + NADH + H+ L-Lactate + NAD+

SPECIFICATION

Appearancewhite lyophilizate
Activity≧150 U/mg lyophilizate
ContaminantsGPT ≦1.0×10-2
malate dehydrogenase ≦5.0×10-2
pyruvate kinase ≦1.0×10-2
Storagebelow -20℃

PROPERTIES

Molecular weightca. 115 kDa (gel filtration)
Michaelis constant2.5×10-2M (fructosyl-glycine)
1.0×10-4M (pyruvate)
pH Optimum7.4–8.0
pH Stability7.0–7.5
Optimum temperature50–55℃
Thermal stabilitybelow 45℃

APPLICATIONS

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

REFERENCES

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).

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