Creatinase (C2-AT)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

Origin recombinant E. coli
Systematic name

Creatine amidinohydrolase

EC Number 3.5.3.3
Reaction formula

Creatine + H2O →→→ Sarcosine + Urea

SPECIFICATION

Appearance white lyophilizate
Activity ≧9 U/mg lyophilizate
Contaminant catalase <0.5%
Stabilizer sucrose
Storage below -20℃

PROPERTIES

Molecular weight ca. 80 kDa (gel filtration)
Structure 2 subunits of 48 kDa (SDS-PAGE)
Michaelis constant 8.6×10-3M (creatine)
pH Optimum 7.0–9.0
pH Stability 4.0–11.0
Optimum temperature 45℃
Thermal stability below 53℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Hg2+

APPLICATIONS

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

REFERENCES

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

TOP