Maltose Phosphorylase (MPL-EP)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

Origin recombinant E. coli
Lineage name

Maltose : orthophosphate 1-β-D-glucosyltransferase

EC Number 2.4.1.8
Reaction formula

Maltose + Orthophosphate →→→ D-Glucose + β-D-Glucose 1-phosphate

SPECIFICATION

Appearance white lyophilizate
Activity ≧10 U/mg lyophilizate
Contaminants NADH oxidase ≦5.0×10-4
6-phosphogluconate dehydrogenase ≦2.0×10-4
Stabilizer lactose,EDTA
Storage below -20℃

PROPERTIES

Molecular weight

ca. 220 kDa (gel filtration)

Structure 2 subunits of 90 kDa (SDS-PAGE)
Michaelis constant

1.9×10-3M (phosphate)

3.4×10-3 M (phosphate)

8.3×10-3M (arsenate)

pH Optimum 6.5–7.5
pH Stability 5.5–8.0
Optimum temperature 45–50°C
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at least four weeks
Inhibitors

Hg2+,Ag+,Zn2+,Cu2+

APPLICATIONS

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

REFERENCES

  • Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).
  • Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).

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