Cholesterol Oxidase (CHO-CE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of cholesterol in clinical analysis.

Origin recombinant E. coli
Systematic name

Cholesterol : oxygen oxidoreductase

EC Number 1.1.3.6
Reaction formula

Cholesterol + O2 →→→ Cholest-4-en-3-one + H2O2

SPECIFICATION

Appearanceyellow lyophilizate
Activity≧5 U/mg lyophilizate
Contaminantscatalase ≦2.0%
cholesterol esterase ≦1.0×10-2%
Stabilizertrehalose,glutamate, malate, EDTA
Storagebelow -20℃ protected from light

PROPERTIES

Molecular weightca. 58 kDa (gel filtration)
Structuremonomer of 55 kDa (SDS-PAGE)
Michaelis constant3.5×10-4 M (cholesterol)
pH Optimum7.0
pH Stability5.0–10.0
Optimum temperature50℃
Thermal stabilitybelow 55℃
Stability (liquid form)stable at 37℃ for at least one week
Stability (powder form)stable at 30℃ for at least one month
Inhibitorsionic detergents, Ag+, Hg2+

APPLICATIONS

The enzyme is useful for the determination of cholesterol in clinical analysis.

REFERENCES

  • Shirokane, Y. et al., J. Ferment. Technol., 55, 337–346 (1977).
  • Richmond, W., Clin. Chem., 19, 1350–1356 (1973).
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79–84 (1973).
  • Allain, C. C. et al., Clin. Chem., 20, 470–475 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724–725 (1974).

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