Fructosyl-peptide Oxidase (FPOX-CET)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

Origin recombinant E. coli
Systematic name

Fructosyl-peptide : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

SPECIFICATION

Appearanceyellow lyophilizate
Activity≧5.0 U/mg lyophilizate
Stabilizerglutamate, EDTA
Storagebelow -20℃ protected from light

PROPERTIES

Molecular weightca. 60 kDa (gel filtration)
Structuremonomer of 52 kDa(SDS-PAGE)
Michaelis constant1.5×10-3M(fructosyl-valyl-histidine)
5.0×10-3M (fructosyl-glysine)
9.0×10-3M(Nε-fructosyl-lysine)
pH Optimum7.5–8.5
pH Stability5.5–9.5
Optimum temperature37–45℃
Thermal stabilitybelow 45℃
Stability (powder form)stable at -20℃ for at least 12 months
InhibitorsHg2+ ,Pb2+
Specificityfructosyl-valyl-histidine (100), fructosyl-glycine(150)
Nε-fructosyl-L-lysine (68.6)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

REFERENCES

  • Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104-111 (2003).
  • Hirokawa, K. et al., Appl Microbiol Biotechnol., 78, 775-781 (2008).

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