alpha-Glucosidase (aGLS-SE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of α-amylase and serum chloride in clinical analysis.

Origin recombinant E. coli
Lineage name

α-D-Glucoside glucohydrolase

EC Number 3.2.1.20
Reaction formula

α-D-Glucoside + H2O →→→ α-D-Glucose + ROH

SPECIFICATION

Appearance white lyophilizate
Activity ≧10 U/mg lyophilizate
Contaminant α-amylase  ≦5.0×10-3
Stabilizer albumin from bovine serum
Storage below -20℃

PROPERTIES

Molecular weight ca. 61 kDa (gel filtration)
Structure monomer of 60 kDa (SDS-PAGE)
Michaelis constant 5.5×10-5M (p-nitrophenyl-α-D-glucoside)
pH Optimum 6.0–9.0
pH Stability 5.0–10.0
Optimum temperature 52–55°C
Thermal stability below 50℃
Stability (liquid form) stable at 37℃ for at least two weeks
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Hg2+,Ag+
Specificity 下記表組参照
Specificity
Substrate*a Relative activity (%)*b
PNPG 100.0
PNPG2 9.4
PNPG5 1.1
Maltose 10.2
Maltotriose 112.9
Maltotetraose 13.9
Maltopentaose 1.4

a:Substrate concentration, 2.2 mM

b:Glucose-forming activity, pH 7.0 at 37℃

APPLICATIONS

The enzyme is useful for the determination of α-amylase and serum chloride in clinical analysis.

REFERENCES

  • Halvorson, H., “Methods in Enzymology,” Vol. 8, Academic Press, New York, 1966, pp. 559–562.
  • Ono, T. et al., Clin. Chem., 34, 552–553 (1988).

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