Glucose Dehydrogenase (FADGDH-AB)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

Systematic name

D-Glucose : acceptor 1-oxidoreductase

Reaction formula

D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor

SPECIFICATION

Appearance Yellow lyophilizate
Activity ≧700 U/mg lyophilizate
Contaminant NAD Glucose Dehydrogenase  ≺1.0×10-2
Hexokinase ≺1.0×10-2
α-Glucosidase ≺1.0×10-2
β-Glucosidase ≺1.0×10-2
Storage below -20℃ protected from light

PROPERTIES

Molecular weight ca. 85 kDa (SDS-PAGE)
Structure monomer, one mole of FAD per mole of enzyme glycoprotein
Michaelis constant 1.1×10-1M (D-Glucose)
pH Optimum 7.0–7.5
pH Stability 4.5–8.0
Optimum temperature 40–45℃
Thermal stability below 45℃
Specificity D-glucose (100), Maltose (≺0.3), 
D-xylose (≺10), D-galactose (≺1.2) 
sucrose (≺0.0) 

APPLICATIONS

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

REFERENCES

Satake, R. et al., J Biosci Bioeng., 120, 498-503 (2015)

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