臨床検査および血糖自己測定(SMBG)において、D-グルコースの測定に利用されます。
| 由来 | recombinant A. sojae |
|---|---|
| 系統名 | D-Glucose : acceptor 1-oxidoreductase |
| EC 番号 | 1.1.5.9 |
| 反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Glucose Dehydrogenase (FADGDH-AB) グルコースデヒドロゲナーゼ(FADGDH-AB)
- 臨床診断用酵素
仕様
| Appearance | yellow lyophilizate | |
|---|---|---|
| Activity | ≧700 U/mg | |
| Contaminants | NAD glucose dehydrogenase ≺1.0×10-2 U/U％ | |
| Hexokinase ≺1.0×10-2 U/U％ | ||
| α-glucosidase ≺1.0×10-2 U/U％ | ||
| β-glucosidase ≺1.0×10-2 U/U％ | ||
| Filterability | ≧90% | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 85 kDa (SDS-PAGE) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 2.2×10-2M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 4.5–8.0 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 45℃ |
| Specificity | D-glucose (100), maltose (≺0.01), |
| D-xylose (8.7), D-galactose (0.7) , | |
|
sucrose (0.1) ,D-mannnose (3.9), 2-deoxy-d-glucose (52.2) |
アプリケーション
The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
参考文献
Hatada M, Loew N, Inose-Takahashi Y, Okuda-Shimazaki J, Tsugawa W, Mulchandani A, Sode K (2018)
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.
Bioelectrochemistry, 121, 185-190
Okuda-Shimazaki, J, Yoshida H, Sode K (2020)
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes –
Bioelectrochemistry, 132, 107414