Glucose Dehydrogenase (FADGDH-AB) Glucose Dehydrogenase (FADGDH-AB)

  • 臨床診断用酵素

臨床検査および血糖自己測定において、D-グルコースの測定に利用されます。

由来 recombinant A. sojae
系統名

D-Glucose : acceptor 1-oxidoreductase

EC 番号 1.1.5.9
反応式

D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor

仕様

Appearance Yellow lyophilizate
Activity ≧700 U/mg lyophilizate
Contaminant NAD Glucose Dehydrogenase  ≺1.0×10-2
Hexokinase ≺1.0×10-2
α-Glucosidase ≺1.0×10-2
β-Glucosidase ≺1.0×10-2
Storage below -20℃ protected from light

特性

Molecular weight ca. 85 kDa (SDS-PAGE)
Structure monomer, one mole of FAD per mole of enzyme glycoprotein
Michaelis constant 1.1×10-1M (D-Glucose)
pH Optimum 7.0–7.5
pH Stability 4.5–8.0
Optimum temperature 40–45℃
Thermal stability below 45℃
Specificity D-glucose (100), Maltose (≺0.3), 
D-xylose (≺10), D-galactose (≺1.2) 
sucrose (≺0.0) 

アプリケーション

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

参考文献

Hatada M, Loew N, Inose-Takahashi Y, Okuda-Shimazaki J, Tsugawa W, Mulchandani A, Sode K (2018)
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.
Bioelectrochemistry, 121, 185-190

Okuda-Shimazaki, J, Yoshida H, Sode K (2020)
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes –
Bioelectrochemistry, 132, 107414

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