Creatinase (C2-AE) クレアチナーゼ(C2-AE)

  • 臨床診断用酵素

臨床検査において、クレアチンおよびクレアチニンの測定に利用されます。

由来 recombinant E. coli
系統名

Creatine amidinohydrolase

EC 番号 3.5.3.3
反応式

Creatine + H2O →→→ Sarcosine + Urea

仕様

Appearance white lyophilizate
Activity ≧9 U/mg lyophilizate
Contaminant catalase       <0.5%
Stabilizer sucrose
Storage below -20℃

特性

Molecular weight ca. 80 kDa (gel filtration)
Structure 2 subunits of 46 kDa (SDS-PAGE)
Michaelis constant 1.3×10-2M (creatine)
pH Optimum 7.0–9.0
pH Stability 5.0–11.0
Optimum temperature 40℃
Thermal stability below 45℃
Stability (liquid form) stable at 37℃ for at least one month
Stability (powder form) stable at 30℃ for at least one month
Inhibitors Hg2+

アプリケーション

The enzyme is useful for the determination of creatinine and creatine in clinical analysis.

参考文献

Suzuki, M. (1979)
クレアチニンの酵素的測定法
Medical Technology, 7, 945–950.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

TOP