The enzyme is useful for the determination of blood glucose.
由来 | recombinant Aspergillus sojae |
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系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
The enzyme is useful for the determination of blood glucose.
由来 | recombinant Aspergillus sojae |
---|---|
系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Appearance | Yellow to brown lyophilizate | |
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Activity | ≧700 U/mg lyophilizate | |
Contaminant | NAD Glucose Dehydrogenase ≺0.01 | |
Hexokinase ≺0.01 | ||
α-Glucosidase ≺0.01 | ||
β-Glucosidase ≺0.01 | ||
Stabilizer | Not included | |
Storage | below -20℃ protected from light |
Molecular weight | ca. 90 kDa (SDS-PAGE) |
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Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
Michaelis constant | 6.4×10-2M (D-Glucose) |
pH Optimum | 7.0–7.5 |
pH Stability | 2.5–9.5 |
Optimum temperature | 45℃ |
Thermal stability (liquid form) | below 60℃ |
Thermal stability (powder form) | stable at 30℃ for at least one month |
Specificity | D-glucose (100%), Maltose (0.2%), D-xylose (0.9%), D-galactose (0.8%) sucrose (<0.1%), D-Mannose (0.4%) 2-Deoxy-D-glucose (23.5%) |
Highly stable FADGDH-AD is useful for the determination of D-glucose in clinical analysis and continuous glucose monitoring meter (CGM) for diabetes patients.
Satake R, Ichiyanagi A, Ichikawa K, Hirokawa K, Araki Y, Yoshimura T, Gomi K (2015)
Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae
Biosci Bioeng., 120, 498-503
Masakari Y, Hara C, Araki Y, Gomi K, Ito K (2020)
Improvement in the thermal stability of Mucor prainii-derived FAD-dependent glucose dehydrogenase via protein chimerization
Enzyme Microb Technol., 132, 109387, doi: 10.1016/j.enzmictec.2019.109387.
https://doi.org/10.1016/j.enzmictec.2019.109387, (cited 2020-07-02)