臨床検査および血糖自己測定(SMBG)において、D-グルコースの測定に利用されます。
| 由来 | recombinant A. sojae |
|---|---|
| 系統名 | D-Glucose : acceptor 1-oxidoreductase |
| EC 番号 | 1.1.5.9 |
| 反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Glucose Dehydrogenase (FADGDH-AA) グルコースデヒドロゲナーゼ(FADGDH-AA)
- 臨床診断用酵素
仕様
| Appearance | yellow lyophilizate |
|---|---|
| Activity | ≧ 475 U/mg |
| Contaminants | NAD glucose dehydrogenase ≺1.0×10-2％ |
| Hexokinase ≺1.0×10-2 U/U% | |
| α-Glucosidase ≺1.0×10-2 U/U% | |
| β-Glucosidase ≺1.0×10-2 U/U% | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 90 kDa (gel filtration) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 9.5×10-2M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 2.5–7.5 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 50℃ |
| Inhibitors | Ag+ |
| Specificity | D-glucose (100), maltose (≺1), |
| D-xylose (≺1), D-galactose (≺1) |
アプリケーション
The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
参考文献
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.