Glucose Dehydrogenase (FADGDH-AA) グルコースデヒドロゲナーゼ(FADGDH-AA)

  • 臨床診断用酵素

臨床検査および血糖自己測定において、D-グルコースの測定に利用されます。

由来 recombinant A. sojae
系統名

D-Glucose : acceptor 1-oxidoreductase

EC 番号 1.1.5.9
反応式

D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor

仕様

Appearance Yellow lyophilizate
Activity ≧500 U/mg lyophilizate
Contaminant NAD Glucose Dehydrogenase  ≺1.0×10-2
Hexokinase ≺1.0×10-2
α-Glucosidase ≺1.0×10-2
β-Glucosidase ≺1.0×10-2
Storage below -20℃ protected from light

特性

Molecular weight ca. 90 kDa (gel filtration)
Structure monomer, one mole of FAD per mole of enzyme glycoprotein
Michaelis constant 9.5×10-2M (d-Glucose)
pH Optimum 7.0–7.5
pH Stability 2.5–7.5
Optimum temperature 40–50℃
Thermal stability below 50℃
Inhibitors Ag2+
Specificity d-glucose (100), Maltose (≺1), 
d-xylose (≺1), d-galactose (≺1) 

アプリケーション

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

参考文献

Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)

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