臨床検査および血糖自己測定において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
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系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
臨床検査および血糖自己測定において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
---|---|
系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Appearance | Yellow lyophilizate | |
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Activity | ≧500 U/mg lyophilizate | |
Contaminant | NAD Glucose Dehydrogenase ≺1.0×10-2% | |
Hexokinase ≺1.0×10-2% | ||
α-Glucosidase ≺1.0×10-2% | ||
β-Glucosidase ≺1.0×10-2% | ||
Storage | below -20℃ protected from light |
Molecular weight | ca. 90 kDa (gel filtration) |
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Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
Michaelis constant | 9.5×10-2M (d-Glucose) |
pH Optimum | 7.0–7.5 |
pH Stability | 2.5–7.5 |
Optimum temperature | 40–50℃ |
Thermal stability | below 50℃ |
Inhibitors | Ag2+ |
Specificity | D-glucose (100), Maltose (≺1), |
D-xylose (≺1), d-galactose (≺1) |
The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)