Glucose Dehydrogenase (FADGDH-AA) グルコースデヒドロゲナーゼ(FADGDH-AA)

  • 臨床診断用酵素

臨床検査および血糖自己測定において、D-グルコースの測定に利用されます。

由来 recombinant A. sojae
系統名

D-Glucose : acceptor 1-oxidoreductase

EC 番号 1.1.5.9
反応式

D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor

仕様

AppearanceYellow lyophilizate
Activity≧500 U/mg lyophilizate
ContaminantNAD Glucose Dehydrogenase  ≺1.0×10-2
Hexokinase ≺1.0×10-2
α-Glucosidase ≺1.0×10-2
β-Glucosidase ≺1.0×10-2
Storagebelow -20℃ protected from light

特性

Molecular weightca. 90 kDa (gel filtration)
Structuremonomer, one mole of FAD per mole of enzyme glycoprotein
Michaelis constant9.5×10-2M (d-Glucose)
pH Optimum7.0–7.5
pH Stability2.5–7.5
Optimum temperature40–50℃
Thermal stabilitybelow 50℃
InhibitorsAg2+
SpecificityD-glucose (100), Maltose (≺1), 
D-xylose (≺1), d-galactose (≺1) 

アプリケーション

The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.

参考文献

Satake, R. et al., J Biosci Bioeng., 120, 498–503 (2015)

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