Catalase (CAT)

  • Enzymes for Clinical Chemistry

The enzyme is useful for removal of hydrogen peroxide in clinical analysis.

Origin bovine liver
Lineage name

Hydrogen-peroxide : hydrogen-peroxide oxidoreductase

EC Number 1.11.1.6
Reaction formula

2H2 + O2→→→ O2 + 2H2O

SPECIFICATION

Appearance yellowish green lyophilizate
Activity ≧12,000 U/mg lyophilizate
Stabilizer lactitol
Storage below -20℃

PROPERTIES

Molecular weight ca. 240 kDa (gel filtration)
Structure 4 identical tetrahedrally arranged subunits of ca. 60 kDa each,
4 ferriprotoporphyrin groups per mole of enzyme
pH Optimum 6.5–8.0
pH Stability 4.0–9.5
Optimum temperature ca. 30℃
Thermal stability below 55℃
Inhibitors azide, cyanide, cyanogenbromide, hydroxylamine, ascorbate,nitrite, fluoride, acetate, formate, ethanol, methanol

APPLICATIONS

The enzyme is useful for removal of hydrogen peroxide in clinical analysis.

REFERENCES

  • Nicholls, P. and Schonbaum, G. R., “The Enzyme,” Vol. 8 (2nd ed.), Academic Press, New York and London, 1963, pp. 147–225.
  • Bergmeyer, H. U., “Methods of Enzymatic Analysis,” Vol. 3 (3rd ed.), Verlag Chemie, Weinheim, Germany, 1983, pp. 273–286.

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