Fructosyl-peptide Oxidase (FPOX-CE) 果糖基 - 缩氨酸氧化酶

  • 临床检测用酶

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

由来 recombinant E. coli
系统名称

Fructosyl-peptide : oxygen oxidoreductase

EC编号 1.5.3
反应式

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

规格

Appearance yellow lyophilizate
Activity

≧6.0 U/mg lyophilizate

Stabilizer EDTA, glutamate
Storage below -20℃ protected from light

特性

Molecular weight ca. 60kDa (gel filtration)
Structure monomer of 52 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 3.4×10-4M (fructosyl-valyl-histidine)
4.4×10-4M (fructosyl-glycine)
8.9×10-4M (Nε-fructosyl-lysine)
pH Optimum 7.5–8.0
pH Stability 6.0–9.5
Optimum temperature 35–42℃
Thermal stability below 45℃
Stability (powder form) stable at 37℃ for at least one month
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

适用类型

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

参考文献

Hirokawa K, Gomi K, Bakke M, Kajiyama N. (2003)
Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi.
Archives of Microbiology, 180, 227-231.

Hirokawa K, Gomi K, Kajiyama N. (2003)
Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein.
Biochem Biophys Res Commun, 311(1), 104-111.

Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.

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