Creatininase (C1-E) 肌酸肝酶

  • 临床检测用酶

The enzyme is useful for the determination of creatinine in clinical analysis.

由来 recombinant E. coli
系统名称

Creatinine amidohydrolase

EC编号 3.5.2.10
反应式

Creatinine H2O Creatine

规格

Appearance yellow lyophilizate
Activity ≧500 U/mg lyophilizate
Contaminant catalase ≦1.0%
Stabilizer sucrose
Storage below -20℃

特性

Molecular weight ca. 170 kDa (gel filtration)
Structure  6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature  60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

适用类型

The enzyme is useful for the determination of creatinine in clinical analysis.

参考文献

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.

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