臨床検査において、α-アミラーゼおよび血中クロライドの測定に利用されます。
| 由来 | recombinant E. coli |
|---|---|
| 系統名 | α-D-Glucoside glucohydrolase |
| EC 番号 | 3.2.1.20 |
| 反応式 | α-D-Glucoside + H2O →→→ α-D-Glucose + ROH |
alpha-Glucosidase (αGLS-SE) α-グルコシダーゼ(αGLS-SE)
- 臨床診断用酵素
仕様
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧10 U/mg | |
| Stabilizer | bovine serum albumin | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 61 kDa (gel filtration) |
|---|---|
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 5.5×10-5M (p-nitrophenyl-α-D-glucoside) |
| pH Optimum | 6.0–9.0 |
| pH Stability | 5.0–10.0 |
| Optimum temperature | 52–55°C |
| Thermal stability | below 50℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg2+，Ag+ |
| Specificity | Refer to the table shown below. |
| Specificity | |
|---|---|
| Substrate*a | Relative activity (%)*b |
| PNPG | 100.0 |
| PNPG2 | 9.4 |
| PNPG5 | 1.1 |
| maltose | 10.2 |
| maltotriose | 112.9 |
| maltotetraose | 13.9 |
| maltopentaose | 1.4 |
a：Substrate concentration, 2.2 mM
b：Glucose-forming activity, pH 7.0 at 37℃
アプリケーション
The enzyme is useful for the determination of α-amylase and serum chloride in clinical analysis.
参考文献
- Halvorson, H., “Methods in Enzymology,” Vol. 8, Academic Press, New York, 1966, pp. 559–562.
- Ono, T. et al., Clin. Chem., 34, 552–553 (1988).