臨床検査において、過酸化水素の除去に利用されます。
| 由来 | bovine liver |
|---|---|
| 系統名 | Hydrogen-peroxide : hydrogen-peroxide oxidoreductase |
| EC 番号 | 1.11.1.6 |
| 反応式 | 2H2 + O2→→→ O2 + 2H2O |
Catalase (CAT) カタラーゼ(CAT)
- 臨床診断用酵素
仕様
| Appearance | yellowish green to brown lyophilizate | |
|---|---|---|
| Activity | ≧12,000 U/mg | |
| Stabilizer | lactitol | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 240 kDa (gel filtration) |
|---|---|
| Structure | 4 identical tetrahedrally arranged subunits of ca. 60 kDa each, 4 ferriprotoporphyrin groups per mole of enzyme |
| pH Optimum | 6.5–8.0 |
| pH Stability | 4.0–9.5 |
| Optimum temperature | ca. 30℃ |
| Thermal stability | below 55℃ |
| Inhibitors | azide, cyanide, cyanogenbromide, hydroxylamine, ascorbate,nitrite, fluoride, acetate, formate, ethanol, methanol |
アプリケーション
The enzyme is useful for removal of hydrogen peroxide in clinical analysis.
参考文献
- Nicholls, P. and Schonbaum, G. R., “The Enzyme,” Vol. 8 (2nd ed.), Academic Press, New York and London, 1963, pp. 147–225.
- Bergmeyer, H. U., “Methods of Enzymatic Analysis,” Vol. 3 (3rd ed.), Verlag Chemie, Weinheim, Germany, 1983, pp. 273–286.