臨床検査において、α-アミラーゼおよび無機リン酸の測定に利用されます。
| 由来 | recombinant E. coli |
|---|---|
| 系統名 | Maltose : orthophosphate 1-β-D-glucosyltransferase |
| EC 番号 | 2.4.1.8 |
| 反応式 | Maltose + Orthophosphate →→→ D-Glucose + β-D-Glucose 1-phosphate |
Maltose Phosphorylase (MPL-EP) マルトースホスホリラーゼ(MPL-EP)
- 臨床診断用酵素
仕様
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧10 U/mg | |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA) | |
| Storage condition | below -20℃ |
特性
| Molecular weight |
ca. 220 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 90 kDa (SDS-PAGE) |
| Michaelis constant |
1.9×10-3M (phosphate) |
|
3.4×10-3 M (phosphate) |
|
|
8.3×10-3M (arsenate) |
|
| pH Optimum | 6.5–7.5 |
| pH Stability | 5.5–8.0 |
| Optimum temperature | 45–50°C |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at least four weeks |
| Inhibitors |
Hg2+，Ag+，Zn2+，Cu2+ |
アプリケーション
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
参考文献
- Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).
- Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).