臨床検査および血糖自己測定(SMBG)において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
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系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
臨床検査および血糖自己測定(SMBG)において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
---|---|
系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Appearance | yellow lyophilizate |
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Activity | ≧ 475 U/mg |
Contaminants | NAD glucose dehydrogenase ≺1.0×10-2% |
Hexokinase ≺1.0×10-2 U/U% | |
α-Glucosidase ≺1.0×10-2 U/U% | |
β-Glucosidase ≺1.0×10-2 U/U% | |
Storage condition | below -20℃ protected from light |
Molecular weight | ca. 90 kDa (gel filtration) |
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Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
Michaelis constant | 9.5×10-2M (D-glucose) |
pH Optimum | 7.0–7.5 |
pH Stability | 2.5–7.5 |
Optimum temperature | 40–50℃ |
Thermal stability | below 50℃ |
Inhibitors | Ag+ |
Specificity | D-glucose (100), maltose (≺1), |
D-xylose (≺1), D-galactose (≺1) |
The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.