3alpha-Hydroxysteroid Dehydrogenase (3aHSD-EH) 3α-ヒドロキシステロイド デヒドロゲナーゼ(3αHSD-EH)

  • 臨床診断用酵素

臨床検査において、胆汁酸の測定に利用されます。

由来 recombinant B.choshinensis
系統名

3α-Hydroxysteroid : NAD(P)+ oxidoreductase

EC 番号 1.1.1.50
反応式

Androsterone + NAD(P)+ →→→ 5α-Androstane-3,17-dione + NAD(P)H

仕様

Appearancewhite to light yellow lyophilizate
Activity≧30 U/mg
Stabilizertrehalose
Storagebelow -20℃

特性

Molecular weightca. 41 kDa (gel filtration)
Structure2 subunits of 25 kDa (SDS-PAGE)
Michaelis constant2.4×10-5M (androsterone)
3.0×10-6M(NAD)
pH Optimum11.0
pH Stability6.0–10.0
Optimum temperature50–60℃
Thermal stabilitybelow 45℃
Stability (powder form)stable at 37℃ for at least four weeks

アプリケーション

The enzyme is useful for the determination of bile acids in clinical analysis.

参考文献

  • Talalay, P., “The Enzymes,” Vol. 7 (2nd ed.), Academic Press, New York and London, 1963, pp. 177–202.
  • Palmer, R., “Methods in Enzymology,” Vol. 15, Academic Press, New York and London, 1969, pp. 280–288.
  • Möbus, E. and Maser, E., J. Biol. Chem., 273, 30888–30896 (1998).

TOP