Fructosyl-amino Acid Oxidase (FAOX-TE) フルクトシルアミノ酸オキシダーゼ(FAOX-TE)

  • 臨床診断用酵素

臨床検査において、フルクトシル-L-アミノ酸の測定に利用されます。

由来 recombinant E. coli
系統名

Fructosyl-L-amino acid : oxygen oxidoreductase

EC 番号 1.5.3
反応式

Fructosyl-L-amino acid + H2O + O2 →→→ L-Amino acid + Glucosone + H2O2

仕様

Appearance yellow lyophilizate
Activity ≧1.0 U/mg lyophilizate
Stabilizer trehalose
Storage below -20℃ protected from light

特性

Molecular weight ca. 88 kDa (gel filtration)
Structure 2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 5.0×10-4M (fructosyl-glycine)
pH Optimum 7.5–8.5
pH Stability 6.0–10.0
Optimum temperature 40–50℃
Thermal stability below 45℃
Stability (liquid form) stable at 20℃ for at least two weeks
Stability (powder form) stable at 37℃ for at least one month
Inhibitors Hg2+,Pb2+
Specificity fructosyl-glycine (100), fructosyl-L-valine (132)
Nε-fructosyl-L-lysine (0)

アプリケーション

The enzyme is useful for the determination of fructosyl-L-amino acid.

参考文献

Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).

Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).

Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).

Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).

Sakaue, R. et al., Acta Cryst., F61, 196–198 (2005).

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