Fructosyl-amino Acid Oxidase (FAOX-TE) フルクトシルアミノ酸オキシダーゼ(FAOX-TE)

  • 臨床診断用酵素

臨床検査において、フルクトシル-L-アミノ酸の測定に利用されます。

由来 recombinant E. coli
系統名

Fructosyl-L-amino acid : oxygen oxidoreductase

EC 番号 1.5.3
反応式

Fructosyl-L-amino acid + H2O + O2 →→→ L-Amino acid + Glucosone + H2O2

仕様

Appearanceyellow lyophilizate
Activity≧1.0 U/mg lyophilizate
Stabilizertrehalose
Storagebelow -20℃ protected from light

特性

Molecular weightca. 88 kDa (gel filtration)
Structure2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point4.2
Michaelis constant5.0×10-4M (fructosyl-glycine)
pH Optimum7.5–8.5
pH Stability6.0–10.0
Optimum temperature40–50℃
Thermal stabilitybelow 45℃
Stability (liquid form)stable at 20℃ for at least two weeks
Stability (powder form)stable at 37℃ for at least one month
InhibitorsHg2+,Pb2+
Specificityfructosyl-glycine (100), fructosyl-L-valine (132)
Nε-fructosyl-L-lysine (0)

アプリケーション

The enzyme is useful for the determination of fructosyl-L-amino acid.

参考文献

Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).

Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).

Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).

Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).

Sakaue, R. et al., Acta Cryst., F61, 196–198 (2005).

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