Cholesterol Esterase (CHE-XE) コレステロース(CHE-XE)

  • 臨床診断用酵素

臨床検査において、各種コレステロールの測定に利用されます。

由来 recombinant E. coli
系統名

Steryl-ester acylhydrolase

EC 番号 3.1.1.13
反応式

Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid

仕様

Appearance light yellow lyophilizate
Activity ≧5 U/mg lyophilizate
Stabilizer sucrose
Storage below -20℃

特性

Molecular weight ca. 54 kDa (gel filtration)
Structure monomer of 54 kDa (SDS-PAGE)
Michaelis constant 1.9×10-5 M (cholesterol linoleate)
pH Optimum 5.5–7.0
pH Stability 5.0–10.0
Optimum temperature 40℃
Thermal stability below 55℃
Specificity cholesterol linoleate (100), cholesterol acetate (2)
cholesterol oleate (98), cholesterol palmitate (74)
cholesterol stearate (68), cholesterol arachidonate (46)

アプリケーション

The enzyme is useful for the determination of cholesterol in clinical analysis.

参考文献

  • Richmond, W., Clin. Chem., 19, 1350 (1973)
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
  • Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
  • Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).

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