Cholesterol Esterase (CHE-XE) コレステロース(CHE-XE)

  • 臨床診断用酵素

臨床検査において、各種コレステロールの測定に利用されます。

由来 recombinant E. coli
系統名

Steryl-ester acylhydrolase

EC 番号 3.1.1.13
反応式

Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid

仕様

Appearancelight yellow lyophilizate
Activity≧5 U/mg lyophilizate
Stabilizersucrose
Storagebelow -20℃

特性

Molecular weightca. 54 kDa (gel filtration)
Structuremonomer of 54 kDa (SDS-PAGE)
Michaelis constant1.9×10-5 M (cholesterol linoleate)
pH Optimum5.5–7.0
pH Stability5.0–10.0
Optimum temperature40℃
Thermal stabilitybelow 55℃
Specificitycholesterol linoleate (100), cholesterol acetate (2)
cholesterol oleate (98), cholesterol palmitate (74)
cholesterol stearate (68), cholesterol arachidonate (46)

アプリケーション

The enzyme is useful for the determination of cholesterol in clinical analysis.

参考文献

  • Richmond, W., Clin. Chem., 19, 1350 (1973)
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
  • Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
  • Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).

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