3alpha-Hydroxysteroid Dehydrogenase (3aHSD-EH)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of bile acids in clinical analysis.

Origin recombinant B.choshinensis
Systematic name

3α-Hydroxysteroid : NAD(P)+ oxidoreductase

EC Number 1.1.1.50
Reaction formula

Androsterone + NAD(P)+ →→→ 5α-Androstane-3,17-dione + NAD(P)H

SPECIFICATION

Appearancewhite lyophilizate
Activity≧30 U/mg
Stabilizertrehalose
Storagebelow-20℃

PROPERTIES

Molecular weightca. 41 kDa (gel filtration)
Structure2 subunits of 25 kDa (SDS-PAGE)
Michaelis constant2.4×10-5M (androsterone)
3.0×10-6M(NAD)
pH Optimum11.0
pH Stability6.0–10.0
Optimum temperature50–60℃
Thermal stabilitybelow 45℃
Stability (powder form)stable at 37℃ for at least four weeks

APPLICATIONS

The enzyme is useful for the determination of bile acids in clinical analysis.

REFERENCES

  • Talalay, P., “The Enzymes,” Vol. 7 (2nd ed.), Academic Press, New York and London, 1963, pp. 177–202.
  • Palmer, R., “Methods in Enzymology,” Vol. 15, Academic Press, New York and London, 1969, pp. 280–288.
  • Möbus, E. and Maser, E., J. Biol. Chem., 273, 30888–30896 (1998).

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