Fructosyl-amino Acid Oxidase (FAOX-TE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of fructosyl-L-amino acid.

Origin recombinant E. coli
Lineage name

Fructosyl-L-amino acid : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ L-Amino acid + Glucosone + H2O2

SPECIFICATION

Appearance yellow lyophilizate
Activity ≧1.0 U/mg lyophilizate
Stabilizer trehalose
Storage below -20℃ protected from light

PROPERTIES

Molecular weight ca. 88 kDa (gel filtration)
Structure 2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 5.0×10-4M (fructosyl-glycine)
pH Optimum 7.5–8.5
pH Stability 6.0–10.0
Optimum temperature 40–50℃
Thermal stability below 45℃
Stability (liquid form) stable at 20℃ for at least two weeks
Stability (powder form) stable at 37℃ for at least one month
Inhibitors Hg2+,Pb2+
Specificity fructosyl-glycine (100), fructosyl-L-valine (132)
Nε-fructosyl-L-lysine (0)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-L-amino acid.

REFERENCES

Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).

Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).

Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).

Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).

Sakaue, R. et al., Acta Cryst., F61, 196–198 (2005).

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