Fructosyl-amino Acid Oxidase (FAOX-TE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of fructosyl-L-amino acid.

Origin recombinant E. coli
Systematic name

Fructosyl-L-amino acid : oxygen oxidoreductase

EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2 →→→ L-Amino acid + Glucosone + H2O2

SPECIFICATION

Appearanceyellow lyophilizate
Activity≧1.0 U/mg lyophilizate
Stabilizertrehalose
Storagebelow -20℃ protected from light

PROPERTIES

Molecular weightca. 88 kDa (gel filtration)
Structure2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point4.2
Michaelis constant5.0×10-4M (fructosyl-glycine)
pH Optimum7.5–8.5
pH Stability6.0–10.0
Optimum temperature40–50℃
Thermal stabilitybelow 45℃
Stability (liquid form)stable at 20℃ for at least two weeks
Stability (powder form)stable at 37℃ for at least one month
InhibitorsHg2+,Pb2+
Specificityfructosyl-glycine (100), fructosyl-L-valine (132)
Nε-fructosyl-L-lysine (0)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-L-amino acid.

REFERENCES

Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).

Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).

Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).

Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).

Sakaue, R. et al., Acta Cryst., F61, 196–198 (2005).

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