Cholesterol Esterase (CHE-XE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of cholesterol in clinical analysis.

Origin recombinant E. coli
Lineage name

Steryl-ester acylhydrolase

EC Number 3.1.1.13
Reaction formula

Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid

SPECIFICATION

Appearance light yellow lyophilizate
Activity ≧5 U/mg lyophilizate
Stabilizer sucrose
Storage below -20℃

PROPERTIES

Molecular weight ca. 54 kDa (gel filtration)
Structure monomer of 54 kDa (SDS-PAGE)
Michaelis constant 1.9×10-5 M (cholesterol linoleate)
pH Optimum 5.5–7.0
pH Stability 5.0–10.0
Optimum temperature 40℃
Thermal stability below 55℃
Specificity cholesterol linoleate (100), cholesterol acetate (2)
cholesterol oleate (98), cholesterol palmitate (74)
cholesterol stearate (68), cholesterol arachidonate (46)

APPLICATIONS

The enzyme is useful for the determination of cholesterol in clinical analysis.

REFERENCES

  • Richmond, W., Clin. Chem., 19, 1350 (1973)
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
  • Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
  • Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).

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