Cholesterol Esterase (CHE-XE)

  • Enzymes for Clinical Chemistry

The enzyme is useful for the determination of cholesterol in clinical analysis.

Origin recombinant E. coli
Systematic name

Steryl-ester acylhydrolase

EC Number 3.1.1.13
Reaction formula

Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid

SPECIFICATION

Appearancelight yellow lyophilizate
Activity≧5 U/mg lyophilizate
Stabilizersucrose
Storagebelow -20℃

PROPERTIES

Molecular weightca. 54 kDa (gel filtration)
Structuremonomer of 54 kDa (SDS-PAGE)
Michaelis constant1.9×10-5 M (cholesterol linoleate)
pH Optimum5.5–7.0
pH Stability5.0–10.0
Optimum temperature40℃
Thermal stabilitybelow 55℃
Specificitycholesterol linoleate (100), cholesterol acetate (2)
cholesterol oleate (98), cholesterol palmitate (74)
cholesterol stearate (68), cholesterol arachidonate (46)

APPLICATIONS

The enzyme is useful for the determination of cholesterol in clinical analysis.

REFERENCES

  • Richmond, W., Clin. Chem., 19, 1350 (1973)
  • Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
  • Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
  • Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
  • Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).

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