Lactate Dehydrogenase (LDH-P) 乳酸脱氢酶

  • 临床检测用酶

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

由来 porcine heart
系统名称

(S )-Lactate : NAD+ oxidoreductase
EC编号 1.1.1.27
反应式

Pyruvate + NADH + H+ L-Lactate + NAD+

规格

Appearance white lyophilizate
Activity ≧150 U/mg
Storage condition below -20℃

特性

Molecular weight ca. 115 kDa (gel filtration)
Michaelis constant 2.5×10-2M (fructosyl-glycine)
1.0×10-4M (pyruvate)
pH Optimum 7.4–8.0
pH Stability 7.0–7.5
Optimum temperature 50–55℃
Thermal stability below 45℃

适用类型

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

参考文献

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).

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