Lactate Dehydrogenase (LDH-P) 乳酸脱氢酶

  • 临床检测用酶

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

由来 porcine heart
系统名称

(S )-Lactate : NAD+ oxidoreductase

EC编号 1.1.1.27
反应式

Pyruvate + NADH + H+ L-Lactate + NAD+

规格

Appearancewhite lyophilizate
Activity≧150 U/mg lyophilizate
ContaminantsGPT ≦1.0×10-2
malate dehydrogenase ≦5.0×10-2
pyruvate kinase ≦1.0×10-2
Storagebelow -20℃

特性

Molecular weightca. 115 kDa (gel filtration)
Michaelis constant2.5×10-2M (fructosyl-glycine)
1.0×10-4M (pyruvate)
pH Optimum7.4–8.0
pH Stability7.0–7.5
Optimum temperature50–55℃
Thermal stabilitybelow 45℃

适用类型

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

参考文献

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).

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