Products information

Food Safety and Environmental Hygiene Monitoring System

CheckLite™ CT150

CheckLite™ CT150

CheckLite™ CT150 detects Coliforms from swab samples within 5.5 hours.

Characteristics of kit

CheckLite™ CT is a rapid detection kit for the presence of total coliforms, using a sensitive bioluminometric assay for beta-galactosidase in coliform bacteria.

  • · More rapid detection method than colorimetric assays or fluorescent assays.
  • · Detection for presence or absence of coliform bacteria (not quantitative)

Table. lncubation time required for detecting coliforms★

Strain Incubation time
Escherichia coli IAM12119
Citrobacter freundii JCM1657
Enterobacter cloacae IAM12349
5h
6h
7h

★Initial coliforms per test medium: about 10 colony forming units

Procedure

  • 1

  • 2

  • 3

  • 5

  • 6

Principle of Measurement

  • · CheckLite™ CT150 is an enzymatic presence-absence test for total coliforms relying on the detection of beta-galactosidase. The "Detection reagent CT" contains D-luciferin-O-beta-galacTOPyranoside (LuGal) as an indicator of beta-galactosidase activity, and luciferase.
  • · Luciferin produced from LuGal by beta-galactosidase can be detected as luminescence using luciferase. The amount of bioluminescence is direct proportion to the amount of beta-galactosidase from coliform bacteria in the sample culture.
  • · A highly sensitive bioluminometric assay of beta-galactosidase allows more rapid detection of coliforms than colorimetric assays.

CheckLite™ CT150 should be stored at 2-8 C in a refrigerator. DO NOT FREEZE

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